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Over the years, Sashi Papu John has taught a diverse array of biology courses, ranging from molecular biology to anatomy and physiology, providing students with a comprehensive understanding of biological principles and practices.

Her research expertise spans several areas of biology at the University of Tennessee and the University of Louisville, focusing on molecular and reproductive endocrinology, renal vascular pathophysiology, diabetic nephropathy, and clinical translational research in cancer biology, including studies on lung, breast, prostate, bladder, and colon cancers. Her work has led to several publications in renowned scientific journals and has contributed to advancements in translational research, as well as other areas of biology. She has mentored undergraduate, graduate, Ph.D., and medical students in molecular research at the University of Louisville.